Bacterial community properties are critical to the operation of anaerobic digestion of sewage in Singapore and the relationship between the bacterial community and the plant parameters is essential for optimising process performance in different full-scale sewage sludge digesters in Singapore.
Anaerobic digestion (AD) is a complex biological system that is heavily influenced by different parameters such as solids retention time (SRT), temperature, and substrate characteristics. This is a long-term study of the microbial community and functional gene dynamics associated with process performance in different full-scale sewage sludge digesters in Singapore. Metagenomic and transcriptomic studies targeting taxonomic and functional indicators are periodically conducted and combined with process operation data in a systems biology approach. Laboratory-scale experiments explore the relationship between SRT, microbial functions and AD process performance.